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Santa Cruz Biotechnology rabbit α stat4
Sequences of primers, probes, and siRNA oligonucleotides used F, forward; R, reverse; FAM, carboxyfluorescein ; TAMRA, tetramethylrhodamine.

Sequences of primers, probes, and siRNA oligonucleotides used F, forward; R, reverse; FAM, carboxyfluorescein ; TAMRA, tetramethylrhodamine.

Rabbit α Stat4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit α stat4/product/Santa Cruz Biotechnology
Average 93 stars, based on 283 article reviews

rabbit α stat4 - by Bioz Stars, 2026-05

93/100 stars

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1) Product Images from "Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation * "

Article Title: Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.361709

Figure Legend Snippet: Sequences of primers, probes, and siRNA oligonucleotides used F, forward; R, reverse; FAM, carboxyfluorescein ; TAMRA, tetramethylrhodamine.

Techniques Used: Sequencing

Expression of PIM kinases in Th cells is regulated by the knockdown of STAT4 and STAT6. A , expression of PIM1, PIM2, and PIM3 during human Th cell differentiation. Naïve CD4+ Thp cells isolated from cord blood were activated (Th0) or stimulated with IL-12 (Th1) or IL-4 (Th2). Samples were harvested for Western blotting at the indicated time points. The panels show representative data from four biological replicates. B , expression of PIM1, PIM2, and PIM3 in activated human peripheral CXCR3+ and CXCR3− CD4+ T cells. CD4+ cells were isolated from buffy coats, and the CXCR3-positive and -negative populations were isolated by flow cytometric cell sorting. Sorted cells were activated and harvested at the indicated time points. The panels show representative data of five biological replicates. C , CD4+ T cells were nucleofected with STAT4 (pooled siRNAs 1 + 2) or NT siRNA and polarized in the Th1 direction 20–24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from three biological replicates. D , CD4+ T cells were nucleofected with STAT6 or NT siRNA and polarized in the Th2 direction 24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from two biological replicates. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. d , days.

Figure Legend Snippet: Expression of PIM kinases in Th cells is regulated by the knockdown of STAT4 and STAT6. A , expression of PIM1, PIM2, and PIM3 during human Th cell differentiation. Naïve CD4+ Thp cells isolated from cord blood were activated (Th0) or stimulated with IL-12 (Th1) or IL-4 (Th2). Samples were harvested for Western blotting at the indicated time points. The panels show representative data from four biological replicates. B , expression of PIM1, PIM2, and PIM3 in activated human peripheral CXCR3+ and CXCR3− CD4+ T cells. CD4+ cells were isolated from buffy coats, and the CXCR3-positive and -negative populations were isolated by flow cytometric cell sorting. Sorted cells were activated and harvested at the indicated time points. The panels show representative data of five biological replicates. C , CD4+ T cells were nucleofected with STAT4 (pooled siRNAs 1 + 2) or NT siRNA and polarized in the Th1 direction 20–24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from three biological replicates. D , CD4+ T cells were nucleofected with STAT6 or NT siRNA and polarized in the Th2 direction 24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from two biological replicates. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. d , days.

Techniques Used: Expressing, Knockdown, Cell Differentiation, Isolation, Western Blot, FACS

PIM kinases regulate the IL-12R β 2 / STAT4 pathway in Th1-polarized cells. CD4+ cells were nucleofected and cultured as in . A , samples were harvested at different time points during the culture for TaqMan RT-PCR analysis. These graphs represent the average -fold differences ±S.E. ( error bars ) of IL-12R β 2 mRNA levels in PIM siRNA-treated Th1 cells compared with NT siRNA-treated Th1 cells. Data were calculated from five of six independent experiments. B , expression of the cell surface IL-12Rβ2 protein was analyzed by immunofluorescent staining and flow cytometry at the indicated time points. The bars represent the average -fold changes ±S.E. ( error bars ) of the geometric mean intensities of IL-12Rβ2 in PIM siRNA samples compared with control samples. The data were calculated from six independent experiments. The histograms show representative data from these experiments ( red line , NT siRNA Th1; blue line , PIM123 siRNA Th1; gray lines , isotype controls). Samples were harvested for Western blotting ( C ) and TaqMan RT-PCR ( D ) analyses at the indicated time points. The bars represent the mean values ±S.E. ( error bars ) of the relative levels of STAT4 between control and PIM siRNA samples obtained by quantifying and normalizing against the levels of β-actin. The value of control samples was set as 1. The data were calculated from four independent experiments. The Western blot of STAT1 shows representative data from four independent experiments. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. A–D , statistical significances were calculated using the two-tailed paired t test: *, p < 0.05; **, p < 0.02; ***, p > 0.01 ( A , C , and D ); *, p < 0.005; **, p < 0.0005 ( B ). NT indicates control siRNA. Rel. prot. , relative protein; PerCP , peridinin-chlorophyll protein complex.

Figure Legend Snippet: PIM kinases regulate the IL-12R β 2 / STAT4 pathway in Th1-polarized cells. CD4+ cells were nucleofected and cultured as in . A , samples were harvested at different time points during the culture for TaqMan RT-PCR analysis. These graphs represent the average -fold differences ±S.E. ( error bars ) of IL-12R β 2 mRNA levels in PIM siRNA-treated Th1 cells compared with NT siRNA-treated Th1 cells. Data were calculated from five of six independent experiments. B , expression of the cell surface IL-12Rβ2 protein was analyzed by immunofluorescent staining and flow cytometry at the indicated time points. The bars represent the average -fold changes ±S.E. ( error bars ) of the geometric mean intensities of IL-12Rβ2 in PIM siRNA samples compared with control samples. The data were calculated from six independent experiments. The histograms show representative data from these experiments ( red line , NT siRNA Th1; blue line , PIM123 siRNA Th1; gray lines , isotype controls). Samples were harvested for Western blotting ( C ) and TaqMan RT-PCR ( D ) analyses at the indicated time points. The bars represent the mean values ±S.E. ( error bars ) of the relative levels of STAT4 between control and PIM siRNA samples obtained by quantifying and normalizing against the levels of β-actin. The value of control samples was set as 1. The data were calculated from four independent experiments. The Western blot of STAT1 shows representative data from four independent experiments. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. A–D , statistical significances were calculated using the two-tailed paired t test: *, p < 0.05; **, p < 0.02; ***, p > 0.01 ( A , C , and D ); *, p < 0.005; **, p < 0.0005 ( B ). NT indicates control siRNA. Rel. prot. , relative protein; PerCP , peridinin-chlorophyll protein complex.

Techniques Used: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Flow Cytometry, Control, Western Blot, Two Tailed Test

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( A ) Publicly available chromatin immunoprecipitation sequencing (ChIP-Seq) data for STAT1 (GSM994528), STAT4 (GSM550303), and T-bet (GSM836124) were examined at Cxcr3 . Sequencing tracks were viewed using the Integrative Genomics Viewer (IGV). Regulatory regions of interest with transcription factor enrichment are indicated by the blue boxes. ( B ) Published RNA-Seq data (GSE203065) from in vitro–generated WT and Ikzf3 –/– Th1 cells were analyzed for DEGs. A heatmap of DEGs associated with IFN-γ/STAT1 and IL-12/STAT4 signaling in Th1 cells is shown, as well as additional genes involved in both pathways and Th cell differentiation. Gene names color-coded in blue are downregulated in Ikzf3 –/– Th1 cells. Note: Cxcr3 transcript data presented here are the same as in . ( C ) Schematic of proposed model in which Aiolos may regulate CXCR3 via impacts on components of the IFN-γ/STAT1 and IL-12/STAT4 cytokine signaling pathways. The downward arrows in blue indicate genes that are downregulated in the absence of Aiolos.

Journal: JCI Insight

Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling

doi: 10.1172/jci.insight.180287

Figure Lengend Snippet: ( A ) Publicly available chromatin immunoprecipitation sequencing (ChIP-Seq) data for STAT1 (GSM994528), STAT4 (GSM550303), and T-bet (GSM836124) were examined at Cxcr3 . Sequencing tracks were viewed using the Integrative Genomics Viewer (IGV). Regulatory regions of interest with transcription factor enrichment are indicated by the blue boxes. ( B ) Published RNA-Seq data (GSE203065) from in vitro–generated WT and Ikzf3 –/– Th1 cells were analyzed for DEGs. A heatmap of DEGs associated with IFN-γ/STAT1 and IL-12/STAT4 signaling in Th1 cells is shown, as well as additional genes involved in both pathways and Th cell differentiation. Gene names color-coded in blue are downregulated in Ikzf3 –/– Th1 cells. Note: Cxcr3 transcript data presented here are the same as in . ( C ) Schematic of proposed model in which Aiolos may regulate CXCR3 via impacts on components of the IFN-γ/STAT1 and IL-12/STAT4 cytokine signaling pathways. The downward arrows in blue indicate genes that are downregulated in the absence of Aiolos.

Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), α-Aiolos (1:20,000; 39293, Active Motif), α–β-actin–HRP (1:15,000; A00730, GenScript), goat α-mouse (1:5,000; 115-035-174, Jackson Immunoresearch), and mouse α-rabbit (1:5,000–1:10,000; sc-2357, Santa Cruz Biotechnology).

Techniques: ChIP-sequencing, Sequencing, RNA Sequencing, In Vitro, Generated, Cell Differentiation, Protein-Protein interactions

Journal: The Journal of Biological Chemistry

Article Title: Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation *

doi: 10.1074/jbc.M112.361709

Figure Lengend Snippet: Sequences of primers, probes, and siRNA oligonucleotides used F, forward; R, reverse; FAM, carboxyfluorescein ; TAMRA, tetramethylrhodamine.

Article Snippet: Proteins were detected using the following antibodies: mouse α-PIM1 (12H8, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit α-PIM2 (ATLAS Antibodies, Stockholm, Sweden or D1D2, Cell Signaling Technology, Beverly, MA), rabbit α-PIM3 (D17C9, Cell Signaling Technology), mouse α-T-BET (4B10, Santa Cruz Biotechnology), rabbit α-STAT1 p84/p91 (E-23, Santa Cruz Biotechnology), rabbit α-STAT4 (C-20, Santa Cruz Biotechnology), mouse α-STAT6 (BD Biosciences), mouse α-GAPDH (number 5G4, 6C5, HyTest, Turku, Finland), and mouse α-β-actin (Sigma-Aldrich).

Techniques: Sequencing

Journal: The Journal of Biological Chemistry

Article Title: Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation *

doi: 10.1074/jbc.M112.361709

Figure Lengend Snippet: Expression of PIM kinases in Th cells is regulated by the knockdown of STAT4 and STAT6. A , expression of PIM1, PIM2, and PIM3 during human Th cell differentiation. Naïve CD4+ Thp cells isolated from cord blood were activated (Th0) or stimulated with IL-12 (Th1) or IL-4 (Th2). Samples were harvested for Western blotting at the indicated time points. The panels show representative data from four biological replicates. B , expression of PIM1, PIM2, and PIM3 in activated human peripheral CXCR3+ and CXCR3− CD4+ T cells. CD4+ cells were isolated from buffy coats, and the CXCR3-positive and -negative populations were isolated by flow cytometric cell sorting. Sorted cells were activated and harvested at the indicated time points. The panels show representative data of five biological replicates. C , CD4+ T cells were nucleofected with STAT4 (pooled siRNAs 1 + 2) or NT siRNA and polarized in the Th1 direction 20–24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from three biological replicates. D , CD4+ T cells were nucleofected with STAT6 or NT siRNA and polarized in the Th2 direction 24 h after nucleofection. Samples were harvested for Western blotting at the indicated time points. The panels show representative data from two biological replicates. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. d , days.

Techniques: Expressing, Knockdown, Cell Differentiation, Isolation, Western Blot, FACS

Journal: The Journal of Biological Chemistry

Article Title: Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation *

doi: 10.1074/jbc.M112.361709

Figure Lengend Snippet: PIM kinases regulate the IL-12R β 2 / STAT4 pathway in Th1-polarized cells. CD4+ cells were nucleofected and cultured as in . A , samples were harvested at different time points during the culture for TaqMan RT-PCR analysis. These graphs represent the average -fold differences ±S.E. ( error bars ) of IL-12R β 2 mRNA levels in PIM siRNA-treated Th1 cells compared with NT siRNA-treated Th1 cells. Data were calculated from five of six independent experiments. B , expression of the cell surface IL-12Rβ2 protein was analyzed by immunofluorescent staining and flow cytometry at the indicated time points. The bars represent the average -fold changes ±S.E. ( error bars ) of the geometric mean intensities of IL-12Rβ2 in PIM siRNA samples compared with control samples. The data were calculated from six independent experiments. The histograms show representative data from these experiments ( red line , NT siRNA Th1; blue line , PIM123 siRNA Th1; gray lines , isotype controls). Samples were harvested for Western blotting ( C ) and TaqMan RT-PCR ( D ) analyses at the indicated time points. The bars represent the mean values ±S.E. ( error bars ) of the relative levels of STAT4 between control and PIM siRNA samples obtained by quantifying and normalizing against the levels of β-actin. The value of control samples was set as 1. The data were calculated from four independent experiments. The Western blot of STAT1 shows representative data from four independent experiments. Vertical lines represent repositioned gel lanes that are from the same blot and the same exposure. A–D , statistical significances were calculated using the two-tailed paired t test: *, p < 0.05; **, p < 0.02; ***, p > 0.01 ( A , C , and D ); *, p < 0.005; **, p < 0.0005 ( B ). NT indicates control siRNA. Rel. prot. , relative protein; PerCP , peridinin-chlorophyll protein complex.

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Flow Cytometry, Control, Western Blot, Two Tailed Test

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1) Product Images from "Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation * "

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